Java bali sumatra5/27/2023 ![]() This study was conducted to assess and compare aquatic insect biodiversity and community structure in various headwater streams with respect to different land-use types. However, our study also highlights the need to improve DNA barcode reference libraries of Trichoptera for the Oriental region.Ĭiliwung is one of the major rivers flowing from Cisarua (Bogor) to Jakarta. This study provides an example of how molecular approaches can benefit the exploration of hidden diversity in unexplored areas and can be a valuable tool to link life stages. The high diversity of Trichoptera in this area highlights their potential as bioindicators for water quality assessment. ![]() Adults and larvae could be successfully associated in 18 cases across six families. Only five OTUs could be identified to species level by comparing sequences against the BOLD database using BLAST, and four more to the genus level. The molecular delimitation detected 72 to 81 Operational Taxonomic Units (OTU). In addition to morphological identification, we applied four different molecular taxon delimitation approaches (Generalized Mixed Yule Coalescent, Bayesian Poisson Tree Processes, Automatic Barcode Gap Discovery and Assemble Species by Automatic Partitioning) based on DNA barcoding of Cytochrome-C-Oxidase I (COI). To assess the Trichoptera diversity of the area within and around the Mount Halimun Salak National Park (MHSNP or Taman Nasional Gunung Halimun Salak) in West Java (Indonesia), we conducted a molecular-morphological study on Trichoptera diversity using larvae from a benthic survey and adults from hand-netting. Meanwhile, globally increasing anthropogenic stress on freshwater streams also threatens Trichoptera diversity. However, in many areas, caddisflies remain understudied due to lack of taxonomic expertise. ![]() Trichoptera are one of the most diverse groups of freshwater insects worldwide and one of the main bioindicators for freshwater quality.
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